ABSTRACT
Objective To investigate the anti-inflammatory effect of chlorogenic acid( CGA) on BV2 microglia in-fected by herpes simplex virus ( HSV)-1.Methods BV2 cell was stimulated by HSV-1 to create a cell model of vi-rus encephalitis, then CGA was processed with different concentrations. MTT method was used to measure cell via-bility. Real-time quantitative PCR and ELISA were used to detect the content and expression of tumor necrosis fac-tor alpha ( TNF-alpha) and interleukin ( IL)-6. Results After CGA treatment, the cell survival rate significantly improved, and after HSV-1 infected BV2 cells, CGA inhibited the significant increase in mRNA levels of TNF-al-pha and IL-6. In addition, CGA can reduce the release of TNF-alpha and IL-6 induced by HSV in the cells.The mRNAs of TNF-alpha and IL-6 were also significantly inhibited by CGA.Conclusions CGA inhibits inflammatory response in HSE.CGA can be used as an anti-inflammatory agent to treat HSE new strategies.
ABSTRACT
<p><b>OBJECTIVE</b>To study the indexes for evaluating intervertebral disc degeneration in rats during the aging process.</p><p><b>METHODS</b>Nine SD rats were fed for 6 months and 12 for 22 months as the young and aged groups, respectively. The Miyamoto's grade of the rats was calculated, and the quantity and relative area of the vascular buds as well as the thickness of the calcified and non-calcified layers of the cartilage endplate were measured using the stereoscopic method. Immunohistochemistry with monoclonal antibodies was used to determine the expressions of collagens II and X in the endplate.</p><p><b>RESULTS</b>The quantity and relative area of the vascular buds, non-calcified layer/calcified layer ratio, type II collagen expression in the calcified layer and nucleus pulposus of the cartilage endplate were all significantly decreased in the aged rats as compared with those of the youth rats (P<0.05), but the collagen X expression in the non-calcified layer was significantly higher in the aged rats (P=0.003). No significant difference was found in the Miyamoto's grade between the aged and young rats (P=1.130).</p><p><b>CONCLUSION</b>The relative area of the vascular buds, non-calcified layer/calcified layer ratio, phenotypic expressions of collagens II and X in the cartilage endplate, but not the Miyamoto's grade, are sensitive indexes for evaluating intervertebral disc degeneration in rats during the aging process.</p>
Subject(s)
Animals , Rats , Aging , Cartilage , Pathology , Collagen Type II , Collagen Type X , Intervertebral Disc Degeneration , Pathology , Rats, Sprague-DawleyABSTRACT
Objective To investigate the preventive effect of TGF-?_1 neutralizing antibody on flexor tendon adhesion from operation.Methods One hundred and eight leghon cocks performed anastomonsis op- eration were divieded into three groups randomly,as normal saline(control group),5?g/ml group,10?g/ml group of TGF-?_1,antibody.At 1 st,3rd,8th and 12th weeks respectively after operation,the flexor biomechan- ics test,HE staining,Masson staining,Sirius red-polarization staining and TGF-?_1 immunohistochemistry stai- ning were used.Results The max of strength of tendon and the stimulate active flexor from the experiment groups(5?g/ml group,10?g/ml) are higher than from the control group,The max of strength of tendon of the experiment groups are less at 8th weeks,and no difference at 12th weeks from the control group;Compared with the control group,the 10?g/ml group were less shorten the progress of inflammation and accelerated the progress of molding;In the experiment groups(5?g/ml group,10?g/ml),the density of the collagenⅠtype were less,the ratio ofⅠ/Ⅲcollagen and expression of the TGF-?_1 were decreasing.Condusion The study showed that applying of TGF-?_1 muhiclonal neutralizing antibody can inhibit efficiently the function of the TGF-?_1 during the flexor tendon repair,reduce tendon adhesion and scar fromation,however has no affec- tion of tendon intensity,suggesting it is a latent and efficient method for preventiong flexor tendon from adhe- ring after operation.